This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-AKAP79-AKAR4
catalog :
181957
citations: 1
Reference
Tenner B, Getz M, Ross B, Ohadi D, Bohrer C, Greenwald E, et al. Spatially compartmentalized phase regulation of a Ca2+-cAMP-PKA oscillatory circuit. elife. 2020;9: pubmed publisher
product information
Catalog Number :
181957
Product Name :
pcDNA3-AKAP79-AKAR4
article :
doi10.7554/eLife.55013
id28224596
pubmed_id33201801
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5409
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Genetically encoded FRET-based PKA activity reporter fused to AKAP79.
origin :
37
pi :
alt_names
AKAP79-Cerulean-FHA1-PKAsub-cpVenus(E172)
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKAP75, AKAP79, H21
geneAKAP5
id9495
genbank_ids
mutation
nameAKAP79-AKAR4
shRNA_sequence
size3330
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHIS tag
locationN terminal on insert
tagAKAP79
locationN terminal on insert
tagCerulean
locationC terminal on insert
tagcpVenus(E172)
plasmid copy :
AKAP79 cDNA was provided by John D. Scott (University of Washington).
resistance markers :
2182
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA