This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps
catalog :
181956
citations: 1
Reference
Tenner B, Getz M, Ross B, Ohadi D, Bohrer C, Greenwald E, et al. Spatially compartmentalized phase regulation of a Ca2+-cAMP-PKA oscillatory circuit. elife. 2020;9: pubmed publisher
product information
Catalog Number :
181956
Product Name :
pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps
article :
doi10.7554/eLife.55013
id28224596
pubmed_id33201801
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5409
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Genetically encoded FRET-based cAMP indicator fused to AKAP79.
origin :
37
pi :
alt_names
AKAP79-Citrine-Epac2B(285-443)-Cerulean
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKAP75, AKAP79, H21
geneAKAP5
id9495
genbank_ids
mutation
nameAKAP79-(Ci/Ce)-Epac2-camps
shRNA_sequence
size3273
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHIS tag
locationN terminal on insert
tagAKAP79
locationN terminal on insert
tagCitrine (YFP)
locationC terminal on insert
tagCerulean (CFP)
plasmid copy :
AKAP79 cDNA was provided by John D. Scott (University of Washington). (Ci/Ce)-Epac2-camps was provided by Dermot M.F. Cooper (University of Cambridge).
resistance markers :
2182
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA