This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pKB083
catalog :
181952
citations: 1
Reference
Butcher R, Tabor J. Real-time detection of response regulator phosphorylation dynamics in live bacteria. Proc Natl Acad Sci U S A. 2022;119:e2201204119 pubmed publisher
product information
Catalog Number :
181952
Product Name :
pKB083
article :
doi10.1073/pnas.2201204119
id28224612
pubmed_id35994658
bacterial resistance :
Spectinomycin
cloning :
backbonep15a
backbone_mutation
backbone_origin
backbone_size1885
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please note: Plasmid contains a S286L mutation in lacI. This mutation is not known to affect plasmid function.
growth strain :
IPTG-inducible expression of PhoQ
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsaI
cloning_site_5BsaI
promoterPhoQ-Ptac; lacI-PlacIq
sequencing_primer_3
sequencing_primer_5
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesb0345, ECK0342
genelacI
id945007
genbank_ids
PhoQ - 1252748; lacI - 945007
mutation
namePhoQ; lacI
shRNA_sequence
size
species
32644
Other
PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli
tags
resistance markers :
1868
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA