This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-CMV-NFE2L2-Puro
catalog :
181919
citations: 1
Reference
Forcina G, Pope L, Murray M, Dong W, Abu Remaileh M, Bertozzi C, et al. Ferroptosis regulation by the NGLY1/NFE2L1 pathway. Proc Natl Acad Sci U S A. 2022;119:e2118646119 pubmed publisher
product information
Catalog Number :
181919
Product Name :
pLenti-CMV-NFE2L2-Puro
article :
doi10.1073/pnas.2118646119
id28224605
pubmed_id35271393
bacterial resistance :
Ampicillin
cloning :
backbonepLenti-CMV-Puro-DEST
backbone_mutation
backbone_origin
backbone_size7999
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Please note: Plasmid contains a 15 base pair deletion at the attB1 site and a 14 base pair deletion at the attB2 site compared to the depositor's provided sequence. These differences are not known to affect plasmid function.
growth strain :
Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.
origin :
37
pi :
alt_names
NRF2
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterSFFV
sequencing_primer_3CATTAAAGCAGCGTATCC
sequencing_primer_5CTCACGGGGATTTCCAAGTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEBP1, IMDDHH, NRF2, Nrf-2
geneNFE2L2
id4780
genbank_ids
mutation
nameNFE2L2
shRNA_sequence
size1818
species
9606
Homo sapiens
tags
resistance markers :
4895
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA