This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-EGFP.T2A.NCLX.3'UTR
catalog :
181872
citations: 1
Reference
Hagenston A, Yan J, Bas Orth C, Tan Y, Sekler I, Bading H. Disrupted expression of mitochondrial NCLX sensitizes neuroglial networks to excitotoxic stimuli and renders synaptic activity toxic. J Biol Chem. 2022;298:101508 pubmed publisher
product information
Catalog Number :
181872
Product Name :
pAAV-EGFP.T2A.NCLX.3'UTR
article :
doi10.1016/j.jbc.2021.101508
id28224600
pubmed_id34942149
bacterial resistance :
Ampicillin
cloning :
backbonepAAV-CAG
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Adenoviral
AAV
growth strain :
Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)
origin :
37
pi :
alt_names
NCLX
sodium/calcium/lithium exchanger
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5AgeI
promotersynthetic hybrid CAG promoter
sequencing_primer_3ACGGGAAGCAATAGCATGATAC
sequencing_primer_5GATCACATGGTCCTGCTG
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesAF261233, NCKX6, NCLX, Slc24a6
geneSlc8b1
id170756
genbank_ids
NM_133221
mutationincludes a large portion of the Slc8b1 3'UTR downstream of the CDS
namesolute carrier family 8 member B1
shRNA_sequence
size1755
species
10090
Mus musculus
tags
locationC terminal on insert
tagmyc
alt_names
enhanced GFP
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promotersynthetic hybrid CAG promoter
sequencing_primer_3
sequencing_primer_5TTCGGCTTCTGGCGTGTGA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameEGFP
shRNA_sequence
size717
species
32644
Other
tags
locationC terminal on insert
tagHA
locationN terminal on insert
tagT2A
alt_names
NCLX 3'UTR
sodium/calcium/lithium exchanger 3'UTR
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5HindIII
promotersynthetic hybrid CAG promoter
sequencing_primer_3ACGGGAAGCAATAGCATGATAC
sequencing_primer_5none
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
NM_133221
mutationthe last 212 nucleotides of the 3'UTR are not included in the sequence
name3'UTR of solute carrier family 8 member B1
shRNA_sequence
size774
species
10090
Mus musculus
tags
plasmid copy :
Israel Sekler, Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
resistance markers :
5817
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA