This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-shNCLX-2
catalog :
181871
citations: 1
Reference
Hagenston A, Yan J, Bas Orth C, Tan Y, Sekler I, Bading H. Disrupted expression of mitochondrial NCLX sensitizes neuroglial networks to excitotoxic stimuli and renders synaptic activity toxic. J Biol Chem. 2022;298:101508 pubmed publisher
product information
Catalog Number :
181871
Product Name :
pAAV-shNCLX-2
article :
doi10.1016/j.jbc.2021.101508
id28224600
pubmed_id34942149
bacterial resistance :
Ampicillin
cloning :
backbonepAAV-U6_CaMKIIa-mCherry
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Mouse Targeting
Adenoviral
AAV
growth strain :
Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
origin :
37
pi :
alt_names
NCLX
sodium/calcium/lithium exchanger
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BamHI
promoterU6
sequencing_primer_3TCACTGAGGAAGCTCTCTG
sequencing_primer_5TTGGGTAGTTTGCAGTTTTAAAATT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAF261233, NCKX6, NCLX, Slc24a6
geneSlc8b1
id170756
genbank_ids
NM_133221
mutation
namesolute carrier 8 family member B1
shRNA_sequenceATGTTGGACTGTGGATCTAAA
size
species
10090
Mus musculus
tags
location
tagmCherry
resistance markers :
5817
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA