This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-shMcu-1
catalog :
181868
citations: 1
Reference
Qiu J, Tan Y, Hagenston A, Martel M, Kneisel N, Skehel P, et al. Mitochondrial calcium uniporter Mcu controls excitotoxicity and is transcriptionally repressed by neuroprotective nuclear calcium signals. Nat Commun. 2013;4:2034 pubmed publisher
product information
Catalog Number :
181868
Product Name :
pAAV-shMcu-1
article :
doi10.1038/ncomms3034
id28224599
pubmed_id23774321
bacterial resistance :
Ampicillin
cloning :
backbonepAAV-U6_CAG-hrGFP
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Mouse Targeting
Adenoviral
AAV
growth strain :
Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter
origin :
37
pi :
alt_names
MCU
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BamHI
promoterU6
sequencing_primer_3TCACTGAGGAAGCTCTCTG
sequencing_primer_5TTGGGTAGTTTGCAGTTTTAAAATT
site_3_destroyed
site_5_destroyed
entrez_gene
aliases2010012O16Rik, C10orf42, Ccdc109a, D130073L02Rik, Gm64
geneMcu
id215999
genbank_ids
NM_001033259.4
mutation
namemitochondrial calcium uniporter
shRNA_sequenceTAGGGAATAAAGGGATCTTA
size
species
10090
Mus musculus
tags
locationN terminal on backbone
taghrGFP
resistance markers :
5817
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA