This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry
catalog :
181851
citations: 1
Reference
Zhang J, Lu T, Stolerman L, Tenner B, Yang J, Zhang J, et al. Phase Separation of a PKA Regulatory Subunit Controls cAMP Compartmentation and Oncogenic Signaling. Cell. 2020;182:1531-1544.e15 pubmed publisher
product information
Catalog Number :
181851
Product Name :
pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry
article :
doi10.1016/j.cell.2020.07.043
id28224582
pubmed_id32846158
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5360
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.
origin :
37
pi :
alt_names
DnaJB1(1-70)-PKAcat(16-351;K72H)-mCherry
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCAFD1, PKACA, PPNAD4
genePRKACA
id5566
aliasesHSPF1, Hdj1, Hsp40, RSPH16B, Sis1
geneDNAJB1
id3337
genbank_ids
mutationLysine 128 in the DnaJB1-PKAcat fusion changed to histidine. Corresponds to K72H in WT PKAcat.
nameDnaJB1-PKAcat(K72H)-mCherry
shRNA_sequence
size1944
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmCherry
resistance markers :
2182
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA