This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pU6-pegRNA-SNCA-A53T
catalog :
181738
citations: 1
Reference
Li H, Busquets O, Verma Y, Syed K, Kutnowski N, Pangilinan G, et al. Highly efficient generation of isogenic pluripotent stem cell models using prime editing. elife. 2022;11: pubmed publisher
product information
Catalog Number :
181738
Product Name :
pU6-pegRNA-SNCA-A53T
article :
doi10.7554/eLife.79208
id28223409
pubmed_id36069759
bacterial resistance :
Ampicillin
cloning :
backbonepU6-pegRNA-GG-acceptor
backbone_mutation
backbone_originDavid Liu (Addgene plasmid # 132777)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells
origin :
37
pi :
SNCA-A53TshRNA_sequen
ceGGAGGGAGTGGTGCATGGTGGTTTTAGA
GCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATC
AACTTGAAAAAGTGGCACCGAGTCGGTGCTTACCTGTTG
TCACACCATGCACCACTCCCs
ize126specie
s
>100r>
Synthetic
<
tr>tags<
/td>
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsaI
cloning_site_5BsaI
promoterHuman U6
sequencing_primer_3
sequencing_primer_5hU6-F (GAGGGCCTATTTCCCATGATT)
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesNACP, PARK1, PARK4, PD1
geneSNCA
id6622
genbank_ids
mutation
namePrime editing pegRNA for
plasmid copy :
backbone: pU6-pegRNA-GG-acceptor from David Liu (Addgene plasmid # 132777)
resistance markers :
5785
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA