This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Lamp1-RFP
catalog :
1817
citations: 58
Reference
Peng W, Schr xf6 der L, Song P, Wong Y, Krainc D. Parkin regulates amino acid homeostasis at mitochondria-lysosome (M/L) contact sites in Parkinson's disease. Sci Adv. 2023;9:eadh3347 pubmed publisher
Minckley T, Salvagio L, Fudge D, VERHEY K, Markus S, Qin Y. Zn2+ decoration of microtubules arrests axonal transport and displaces tau, doublecortin, and MAP2C. J Cell Biol. 2023;222: pubmed publisher
Gallagher E, Holzbaur E. The selective autophagy adaptor p62/SQSTM1 forms phase condensates regulated by HSP27 that facilitate the clearance of damaged lysosomes via lysophagy. Cell Rep. 2023;42:112037 pubmed publisher
Haque M, Emi Y, Sakaguchi M. A conserved WXXE motif is an apical delivery determinant of ABC transporter C subfamily isoforms. Cell Struct Funct. 2023;48:71-82 pubmed publisher
Bai Y, Yu G, Zhou H, Amarasinghe O, Zhou Y, Zhu P, et al. PTP4A2 promotes lysophagy by dephosphorylation of VCP/p97 at Tyr805. Autophagy. 2022;:1-20 pubmed publisher
Kulkarni V, Stempel M, Anand A, Sidibe D, Maday S. Retrograde Axonal Autophagy and Endocytic Pathways Are Parallel and Separate in Neurons. J Neurosci. 2022;42:8524-8541 pubmed publisher
Bonet Ponce L, Cookson M. The endoplasmic reticulum contributes to lysosomal tubulation/sorting driven by LRRK2. Mol Biol Cell. 2022;33:ar124 pubmed publisher
Halcrow P, Kumar N, Quansah D, Baral A, Liang B, Geiger J. Endolysosome Iron Chelation Inhibits HIV-1 Protein-Induced Endolysosome De-Acidification-Induced Increases in Mitochondrial Fragmentation, Mitophagy, and Cell Death. Cells. 2022;11: pubmed publisher
Pathni A, Ozcelikkale A, Rey Suárez I, Li L, Davis S, Rogers N, et al. Cytotoxic T Lymphocyte Activation Signals Modulate Cytoskeletal Dynamics and Mechanical Force Generation. Front Immunol. 2022;13:779888 pubmed publisher
Taniguchi S, Ito Y, Kiritani H, Maruo A, Sakai R, Ono Y, et al. The Ubiquitin Ligase RNF34 Participates in the Peripheral Quality Control of CFTR (RNF34 Role in CFTR PeriQC). Front Mol Biosci. 2022;9:840649 pubmed publisher
Comini M, Sierra Marquez J, Guzman G, Franzen A, Willuweit A, Katona I, et al. CLC Anion/Proton Exchangers Regulate Secretory Vesicle Filling and Granule Exocytosis in Chromaffin Cells. J Neurosci. 2022;42:3080-3095 pubmed publisher
Nagar A, Rahman T, Harton J. The ASC Speck and NLRP3 Inflammasome Function Are Spatially and Temporally Distinct. Front Immunol. 2021;12:752482 pubmed publisher
Hilverling A, Szego E, Dinter E, Cozma D, Saridaki T, Falkenburger B. Maturing Autophagosomes are Transported Towards the Cell Periphery. Cell Mol Neurobiol. 2021;: pubmed publisher
Eisenberg S, Haimov E, Walpole G, Plumb J, Kozlov M, Grinstein S. Mapping the electrostatic profiles of cellular membranes. Mol Biol Cell. 2021;32:301-310 pubmed publisher
Bonet Ponce L, Beilina A, Williamson C, Lindberg E, Kluss J, Saez Atienzar S, et al. LRRK2 mediates tubulation and vesicle sorting from lysosomes. Sci Adv. 2020;6: pubmed publisher
Joshi B, de Beer M, Giepmans B, Zuhorn I. Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes. ACS Nano. 2020;14:4444-4455 pubmed publisher
Espino Saldaña A, Durán Ríos K, Olivares Hernandez E, Rodríguez Ortiz R, Arellano Carbajal F, Martinez Torres A. Temporal and spatial expression of zebrafish mctp genes and evaluation of frameshift alleles of mctp2b. Gene. 2020;738:144371 pubmed publisher
Evans C, Holzbaur E. Degradation of engulfed mitochondria is rate-limiting in Optineurin-mediated mitophagy in neurons. elife. 2020;9: pubmed publisher
Zenko D, Thompson D, Hislop J. Endocytic sorting and downregulation of the M2 acetylcholine receptor is regulated by ubiquitin and the ESCRT complex. Neuropharmacology. 2020;162:107828 pubmed publisher
Minckley T, Zhang C, Fudge D, Dischler A, LeJeune K, Xu H, et al. Sub-nanomolar sensitive GZnP3 reveals TRPML1-mediated neuronal Zn2+ signals. Nat Commun. 2019;10:4806 pubmed publisher
Laflamme C, McKeever P, Kumar R, Schwartz J, Kolahdouzan M, Chen C, et al. Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. elife. 2019;8: pubmed publisher
Jung J, Macalino S, Cui M, Kim J, Kim H, Song D, et al. Transmembrane 4 L Six Family Member 5 Senses Arginine for mTORC1 Signaling. Cell Metab. 2019;29:1306-1319.e7 pubmed publisher
Parker B, Calkin A, Seldin M, Keating M, Tarling E, Yang P, et al. An integrative systems genetic analysis of mammalian lipid metabolism. Nature. 2019;567:187-193 pubmed publisher
Sakai R, Fukuda R, Unida S, Aki M, Ono Y, Endo A, et al. The integral function of the endocytic recycling compartment is regulated by RFFL-mediated ubiquitylation of Rab11 effectors. J Cell Sci. 2019;132: pubmed publisher
Lin A, Gill K, Castañeda M, Matucci I, Eder N, Claxton S, et al. Chemical genetic identification of GAK substrates reveals its role in regulating Na+/K+-ATPase. Life Sci Alliance. 2018;1:e201800118 pubmed publisher
Guedes Dias P, Nirschl J, Abreu N, Tokito M, Janke C, Magiera M, et al. Kinesin-3 Responds to Local Microtubule Dynamics to Target Synaptic Cargo Delivery to the Presynapse. Curr Biol. 2019;29:268-282.e8 pubmed publisher
Cioni J, Lin J, Holtermann A, Koppers M, Jakobs M, Azizi A, et al. Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons. Cell. 2019;176:56-72.e15 pubmed publisher
Ozturk D, Kocak M, Akcay A, Kınoglu K, Kara E, Buyuk Y, et al. MITF-MIR211 axis is a novel autophagy amplifier system during cellular stress. Autophagy. 2018;:1-16 pubmed publisher
Hess K, Oliverio R, Nguyen P, Le D, Ellis J, Kdeiss B, et al. Concurrent action of purifying selection and gene conversion results in extreme conservation of the major stress-inducible Hsp70 genes in mammals. Sci Rep. 2018;8:5082 pubmed publisher
Wong Y, Ysselstein D, Krainc D. Mitochondria-lysosome contacts regulate mitochondrial fission via RAB7 GTP hydrolysis. Nature. 2018;554:382-386 pubmed publisher
Liu P, Tsai K, Hsu C, Tsai W, Cheng J, Chang H, et al. Drug Repurposing Screening Identifies Tioconazole as an ATG4 Inhibitor that Suppresses Autophagy and Sensitizes Cancer Cells to Chemotherapy. Theranostics. 2018;8:830-845 pubmed publisher
Jing H, Zhang X, Wisner S, Chen X, Spiegelman N, Linder M, et al. SIRT2 and lysine fatty acylation regulate the transforming activity of K-Ras4a. elife. 2017;6: pubmed publisher
Clerc P, Jeanjean P, Hallali N, Gougeon M, Pipy B, Carrey J, et al. Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death. J Control Release. 2018;270:120-134 pubmed publisher
Indira D, Varadarajan S, Subhasingh Lupitha S, Lekshmi A, Mathew K, Chandrasekharan A, et al. Strategies for imaging mitophagy in high-resolution and high-throughput. Eur J Cell Biol. 2018;97:1-14 pubmed publisher
Guzman R, Bungert Plümke S, Franzen A, Fahlke C. Preferential association with ClC-3 permits sorting of ClC-4 into endosomal compartments. J Biol Chem. 2017;292:19055-19065 pubmed publisher
Cooney K, Molden B, Kowalczyk N, Russell S, Baldini G. Lipid stress inhibits endocytosis of melanocortin-4 receptor from modified clathrin-enriched sites and impairs receptor desensitization. J Biol Chem. 2017;292:17731-17745 pubmed publisher
Łukasiewicz S, Błasiak E, Szczepanowicz K, Guzik K, Bzowska M, Warszyński P, et al. The interaction of clozapine loaded nanocapsules with the hCMEC/D3 cells - In vitro model of blood brain barrier. Colloids Surf B Biointerfaces. 2017;159:200-210 pubmed publisher
Farías G, Guardia C, De Pace R, Britt D, Bonifacino J. BORC/kinesin-1 ensemble drives polarized transport of lysosomes into the axon. Proc Natl Acad Sci U S A. 2017;114:E2955-E2964 pubmed publisher
Gopal P, Nirschl J, Klinman E, Holzbaur E. Amyotrophic lateral sclerosis-linked mutations increase the viscosity of liquid-like TDP-43 RNP granules in neurons. Proc Natl Acad Sci U S A. 2017;114:E2466-E2475 pubmed publisher
Gornstein E, Schwarz T. Neurotoxic mechanisms of paclitaxel are local to the distal axon and independent of transport defects. Exp Neurol. 2017;288:153-166 pubmed publisher
McLelland G, Lee S, McBride H, Fon E. Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system. J Cell Biol. 2016;214:275-91 pubmed publisher
Fernández B, Fdez E, Gomez Suaga P, Gil F, Molina Villalba I, Ferrer I, et al. Iron overload causes endolysosomal deficits modulated by NAADP-regulated 2-pore channels and RAB7A. Autophagy. 2016;12:1487-506 pubmed publisher
Maday S, Holzbaur E. Compartment-Specific Regulation of Autophagy in Primary Neurons. J Neurosci. 2016;36:5933-45 pubmed publisher
Gupta A, Maccario H, Kriplani N, Leslie N. In Cell and In Vitro Assays to Measure PTEN Ubiquitination. Methods Mol Biol. 2016;1388:155-65 pubmed publisher
Demers Lamarche J, Guillebaud G, Tlili M, Todkar K, Bélanger N, Grondin M, et al. Loss of Mitochondrial Function Impairs Lysosomes. J Biol Chem. 2016;291:10263-76 pubmed publisher
Schwartz V, Friedrich K, Polleichtner G, Gründer S. Acid-sensing ion channel (ASIC) 4 predominantly localizes to an early endosome-related organelle upon heterologous expression. Sci Rep. 2015;5:18242 pubmed publisher
Zhang J, Zhou W, Lin J, Wei P, Zhang Y, Jin P, et al. Autophagic lysosomal reformation depends on mTOR reactivation in H2O2-induced autophagy. Int J Biochem Cell Biol. 2016;70:76-81 pubmed publisher
Noritake K, Aki T, Funakoshi T, Unuma K, Uemura K. Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes. PLoS ONE. 2015;10:e0136952 pubmed publisher
Eitan E, Zhang S, Witwer K, Mattson M. Extracellular vesicle-depleted fetal bovine and human sera have reduced capacity to support cell growth. J Extracell Vesicles. 2015;4:26373 pubmed publisher
Jain S, Farías G, Bonifacino J. Polarized sorting of the copper transporter ATP7B in neurons mediated by recognition of a dileucine signal by AP-1. Mol Biol Cell. 2015;26:218-28 pubmed publisher
Xu J, Chai H, Ehinger K, Egan T, Srinivasan R, Frick M, et al. Imaging P2X4 receptor subcellular distribution, trafficking, and regulation using P2X4-pHluorin. J Gen Physiol. 2014;144:81-104 pubmed publisher
Tsika E, Glauser L, Moser R, Fiser A, Daniel G, Sheerin U, et al. Parkinson's disease-linked mutations in VPS35 induce dopaminergic neurodegeneration. Hum Mol Genet. 2014;23:4621-38 pubmed publisher
Hong W, Amara S. Differential targeting of the dopamine transporter to recycling or degradative pathways during amphetamine- or PKC-regulated endocytosis in dopamine neurons. FASEB J. 2013;27:2995-3007 pubmed publisher
Milde S, Gilley J, Coleman M. Subcellular localization determines the stability and axon protective capacity of axon survival factor Nmnat2. PLoS Biol. 2013;11:e1001539 pubmed publisher
Roberts R, Al Jamal W, Whelband M, Thomas P, Jefferson M, Van den Bossche J, et al. Autophagy and formation of tubulovesicular autophagosomes provide a barrier against nonviral gene delivery. Autophagy. 2013;9:667-82 pubmed publisher
Teng A, Al Montashiri N, Cheng B, Lou P, Ozmizrak P, Chen H, et al. Identification of a phosphorylation-dependent nuclear localization motif in interferon regulatory factor 2 binding protein 2. PLoS ONE. 2011;6:e24100 pubmed publisher
Milev M, Brown C, Mouland A. Live cell visualization of the interactions between HIV-1 Gag and the cellular RNA-binding protein Staufen1. Retrovirology. 2010;7:41 pubmed publisher
Sherer N, Lehmann M, Jimenez Soto L, Ingmundson A, Horner S, Cicchetti G, et al. Visualization of retroviral replication in living cells reveals budding into multivesicular bodies. Traffic. 2003;4:785-801 pubmed
product information
Catalog Number :
1817
Product Name :
Lamp1-RFP
article :
doi10.1034/j.1600-0854.2003.00135.x
id113
pubmed_id14617360
bacterial resistance :
Kanamycin
cloning :
backboneModified Clontech Plasmid
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Lamp-1 sequence contains a D50E mutation, which is not important for function of plasmid. There is also a silent mutation at bp#715 compared to GenBank NM_012857.1
growth strain :
Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.
origin :
37
pi :
alt_names
Lamp-1
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoter
sequencing_primer_3DsRed1-N
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLGP120
geneLamp1
id25328
genbank_ids
mutationD50E (not important for function of plasmid)
namelysosome associated membrane protein 1
shRNA_sequence
size1200
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagRFP
plasmid copy :
Norma Andrews provided Lamp-1
resistance markers :
350
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA