This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Lamp1-YFP
catalog :
1816
citations: 14
Reference
Lee J, Gonzalez Hernandez A, Kristt M, Abreu N, Ro xdf mann K, Arefin A, et al. Distinct beta-arrestin coupling and intracellular trafficking of metabotropic glutamate receptor homo- and heterodimers. Sci Adv. 2023;9:eadi8076 pubmed publisher
Qian Y, Celiker O, Wang Z, Guner Ataman B, Boyden E. Temporally multiplexed imaging of dynamic signaling networks in living cells. Cell. 2023;186:5656-5672.e21 pubmed publisher
Renna F, Enriqu xe9 Steinberg J, Gonzalez C, Manifava M, Tadic M, Orquera T, et al. Ubiquitination Is a Novel Post-Translational Modification of VMP1 in Autophagy of Human Tumor Cells. Int J Mol Sci. 2023;24: pubmed publisher
Cui W, Sathyanarayan A, Lopresti M, Aghajan M, Chen C, Mashek D. Lipophagy-derived fatty acids undergo extracellular efflux via lysosomal exocytosis. Autophagy. 2020;:1-16 pubmed publisher
Laflamme C, McKeever P, Kumar R, Schwartz J, Kolahdouzan M, Chen C, et al. Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. elife. 2019;8: pubmed publisher
Guedes Dias P, Nirschl J, Abreu N, Tokito M, Janke C, Magiera M, et al. Kinesin-3 Responds to Local Microtubule Dynamics to Target Synaptic Cargo Delivery to the Presynapse. Curr Biol. 2019;29:268-282.e8 pubmed publisher
Shih Wei W, Chih Ling C, Kao Y, Martin R, Knölker H, Shiao M, et al. Pentabromopseudilin: a myosin V inhibitor suppresses TGF-? activity by recruiting the type II TGF-? receptor to lysosomal degradation. J Enzyme Inhib Med Chem. 2018;33:920-935 pubmed publisher
Clerc P, Jeanjean P, Hallali N, Gougeon M, Pipy B, Carrey J, et al. Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death. J Control Release. 2018;270:120-134 pubmed publisher
Kang Y, Kirby J. Promotion and Rescue of Intracellular Brucella neotomae Replication during Coinfection with Legionella pneumophila. Infect Immun. 2017;85: pubmed publisher
Ghezzi C, Calmettes G, Morand P, Ribalet B, John S. Real-time imaging of sodium glucose transporter (SGLT1) trafficking and activity in single cells. Physiol Rep. 2017;5: pubmed publisher
Lainšček D, Lebar T, Jerala R. Transcription activator-like effector-mediated regulation of gene expression based on the inducible packaging and delivery via designed extracellular vesicles. Biochem Biophys Res Commun. 2017;484:15-20 pubmed publisher
Ashrafi G, Schlehe J, LaVoie M, Schwarz T. Mitophagy of damaged mitochondria occurs locally in distal neuronal axons and requires PINK1 and Parkin. J Cell Biol. 2014;206:655-70 pubmed publisher
Humphries W, Szymanski C, Payne C. Endo-lysosomal vesicles positive for Rab7 and LAMP1 are terminal vesicles for the transport of dextran. PLoS ONE. 2011;6:e26626 pubmed publisher
Sherer N, Lehmann M, Jimenez Soto L, Ingmundson A, Horner S, Cicchetti G, et al. Visualization of retroviral replication in living cells reveals budding into multivesicular bodies. Traffic. 2003;4:785-801 pubmed
product information
Catalog Number :
1816
Product Name :
Lamp1-YFP
article :
doi10.1034/j.1600-0854.2003.00135.x
id113
pubmed_id14617360
bacterial resistance :
Kanamycin
cloning :
backbonepEYFP-N1
backbone_mutation
backbone_originBD Biosciences / Clontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Lamp-1 sequence contains a D50E mutation, which is not important for function of plasmid. There is also a silent mutation at bp#715 compared to GenBank NM_012857.1
growth strain :
Mammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.
origin :
37
pi :
alt_names
Lamp-1
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoter
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-f
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLGP120
geneLamp1
id25328
genbank_ids
mutationD50E (not important for function of plasmid)
namelysosome associated membrane protein 1
shRNA_sequence
size1200
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagYFP
plasmid copy :
Norma Andrews provided Lamp-1
resistance markers :
350
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA