MSCV h c-MYC IRES GFP | Addgene 18119 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MSCV h c-MYC IRES GFP

catalog :

18119

citations: 9

Reference
Farrington C, Yuan E, Mazhar S, Izadmehr S, Hurst L, Allen Petersen B, et al. Protein phosphatase 2A activation as a therapeutic strategy for managing MYC-driven cancers. J Biol Chem. 2020;295:757-770 pubmed publisher
Mu X, Bai L, Xu Y, Wang J, Lu H. Protein targeting chimeric molecules specific for dual bromodomain 4 (BRD4) and Polo-like kinase 1 (PLK1) proteins in acute myeloid leukemia cells. Biochem Biophys Res Commun. 2019;: pubmed publisher
Lee H, Bao S, Qian Y, Geula S, Leslie J, Zhang C, et al. Stage-specific requirement for Mettl3-dependent m6A mRNA methylation during haematopoietic stem cell differentiation. Nat Cell Biol. 2019;21:700-709 pubmed publisher
Flanagan D, Barker N, Costanzo N, Mason E, Gurney A, Meniel V, et al. Frizzled-7 Is Required for Wnt Signaling in Gastric Tumors with and Without Apc Mutations. Cancer Res. 2019;79:970-981 pubmed publisher
Belur Nagaraj A, Joseph P, Kovalenko O, Wang Q, Xu R, Difeo A. Evaluating class III antiarrhythmic agents as novel MYC targeting drugs in ovarian cancer. Gynecol Oncol. 2018;151:525-532 pubmed publisher
Chia P, Zhong F, Niwa S, Bonnard C, Utami K, Zeng R, et al. A homozygous loss-of-function CAMK2A mutation causes growth delay, frequent seizures and severe intellectual disability. elife. 2018;7: pubmed publisher
Szenker Ravi E, Altunoglu U, Leushacke M, Bosso Lefèvre C, Khatoo M, Thi Tran H, et al. RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6. Nature. 2018;557:564-569 pubmed publisher
Madison J, Zhou F, Nigam A, Hussain A, Barker D, Nehme R, et al. Characterization of bipolar disorder patient-specific induced pluripotent stem cells from a family reveals neurodevelopmental and mRNA expression abnormalities. Mol Psychiatry. 2015;20:703-17 pubmed publisher
Hu Y, Zheng M, Gali R, Tian Z, Topal Görgün G, Munshi N, et al. A novel rapid-onset high-penetrance plasmacytoma mouse model driven by deregulation of cMYC cooperating with KRAS12V in BALB/c mice. Blood Cancer J. 2013;3:e156 pubmed publisher

product information

Catalog Number :

18119

Product Name :

MSCV h c-MYC IRES GFP

article :

doi
id	2127
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

MSCV IRES GFP

backbone_mutation

backbone_origin

Dr. Robert Hawley of George Washington University

backbone_size

5660

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Retroviral

growth notes :

The MSCV vector is described in; Hawley, R.G., Lieu, F.H.L., Fong, A.Z.C., and Hawley, T.S. Versatile retroviral vectors for potential use in gene therapy. Gene Ther. 1: 136-138, 1994. Use of the construct is described in; Park, I., Zhao, R., West, J.A., Yabuuchi, A., Huo, H., Ince, T.A., Lerou, P.H., Lensch, M.W., and Daley, G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 451, 141-146, 2008. Please note that the assembled sequence presented here is not 100% reflective of the actual sequence. See the Addgene diagnostic digest (found under the Resource Information section of the plasmid page) for additional information on the expected plasmid size. Addgene has found another HindIII site at the end of the MYC gene (see our quality control sequence).

origin :

pi :

alt_names

pMIG-cMYC

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

NM_002467

mutation

The CUG which is the start site for Myc I is present, however, there is a 3bp deletion between this and the ATG start site for Myc II.

name

c-MYC

shRNA_sequence

size

1858

species

9606

Homo sapiens