This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
T4 Lysozyme WT
catalog :
18110
citations: 2
Reference
Sharma M, Tyagi J, Poluri K. Quantifying bacterial cell lysis using GFP based fluorimetric assay. Int J Biol Macromol. 2019;138:881-889 pubmed publisher
Kubitza C, Ginsel C, Bittner F, Havemeyer A, Clement B, Scheidig A. T4 lysozyme-facilitated crystallization of the human molybdenum cofactor-dependent enzyme mARC. Acta Crystallogr F Struct Biol Commun. 2018;74:337-344 pubmed publisher
product information
Catalog Number :
18110
Product Name :
T4 Lysozyme WT
article :
doi
id2123
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepHS1403
backbone_mutation
backbone_origin
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. Note that the T4 Lysozyme insert sequence provided by the depositing laboratory differs from GenBank reference sequence NP_049736.1 at two amino acids: R12G and I137R. These differences were confirmed by Addgene's sequencing results.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BamHI
promoter
sequencing_primer_3GGCGTATCACGAGGCCC
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesT4p123
genee
id1258585
genbank_ids
NP_049736
mutation
nameT4 Lysozyme
shRNA_sequence
size600
species
Bacteriophage T4
tags
resistance markers :
316
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA