This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCA528 SPASTIN , residues 112-196
catalog :
180627
citations: 1
Reference
Wenzel D, Mackay D, Skalicky J, Paine E, Miller M, Ullman K, et al. Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission. elife. 2022;11: pubmed publisher
product information
Catalog Number :
180627
Product Name :
pCA528 SPASTIN , residues 112-196
article :
doi10.7554/eLife.77779
id28212032
pubmed_id36107470
bacterial resistance :
Kanamycin
cloning :
backbonepCA528
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2022.02.09.477148v1
growth strain :
Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 term
sequencing_primer_5T7 for
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationResidues 112-196
nameSPASTIN
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHIS-SUMO
resistance markers :
2886
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA