This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
W118-1_hRSK3(RPS6KA2)_K91A_K444A
catalog :
180385
citations: 1
Reference
Lee W, Lavery L, Rousseaux M, Rutledge E, Jang Y, Wan Y, et al. Dual targeting of brain region-specific kinases potentiates neurological rescue in Spinocerebellar ataxia type 1. EMBO J. 2021;40:e106106 pubmed publisher
product information
Catalog Number :
180385
Product Name :
W118-1_hRSK3(RPS6KA2)_K91A_K444A
article :
doi10.15252/embj.2020106106
id28223462
pubmed_id33709453
bacterial resistance :
Ampicillin
cloning :
backboneW118-1
backbone_mutation
backbone_origin
backbone_size8015
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3CATAGCGTAAAAGGAGCAACA
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHU-2, MAPKAPK1C, RSK, RSK3, S6K-alpha, S6K-alpha2, p90-RSK3, p90RSK2, pp90RSK3
geneRPS6KA2
id6196
genbank_ids
mutationK91A/K444A
nameRPS6KA2_K91A/K444A
shRNA_sequence
size2202
species
9606
Homo sapiens
tags
resistance markers :
617
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA