This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MSCV-Arkadia C946S H948L
catalog :
180373
citations: 1
Reference
Xu H, Wu L, Nguyen H, Mesa K, Raghavan V, Episkopou V, et al. Arkadia-SKI/SnoN signaling differentially regulates TGF-β-induced iTreg and Th17 cell differentiation. J Exp Med. 2021;218: pubmed publisher
product information
Catalog Number :
180373
Product Name :
MSCV-Arkadia C946S H948L
article :
doi10.1084/jem.20210777
id28223361
pubmed_id34473197
bacterial resistance :
Ampicillin
cloning :
backboneMSCV-IRES-Thy1.1 DEST
backbone_mutation
backbone_origin
backbone_size6395
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Plasmid contains Arkadia isoform 3 Please note: Plasmid contains a D454N mutation in Arkadia. This mutation is not known to affect plasmid function.
growth strain :
expressing mouse Arkadia (isoform 3) C946S H948L E3 ligase dead mutant proteins
inserts :
Stbl3
origin :
37
pi :
alt_names
RNF111
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI sticky end to 5' of Arkadia and BglII sticky end of MSCV
promoterLTR promoter
sequencing_primer_3GGGGCGGAATTCGATATCAAGCT
sequencing_primer_5CGTCTCTCCCCCTTGAACCT
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesARK, Arkadia
geneRnf111
id93836
genbank_ids
mutationC946S H948L
nameArkadia
shRNA_sequence
size
species
10090
Mus musculus
tags
resistance markers :
295
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA