This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSIN-Arkadia-gRNA3
catalog :
180364
citations: 1
Reference
Xu H, Wu L, Nguyen H, Mesa K, Raghavan V, Episkopou V, et al. Arkadia-SKI/SnoN signaling differentially regulates TGF-β-induced iTreg and Th17 cell differentiation. J Exp Med. 2021;218: pubmed publisher
product information
Catalog Number :
180364
Product Name :
pSIN-Arkadia-gRNA3
article :
doi10.1084/jem.20210777
id28223361
pubmed_id34473197
bacterial resistance :
Ampicillin
cloning :
backbonepSIN
backbone_mutationThe retroviral sgRNA expression vector pSIN-U6-sgRNA-EF1as-Thy1.1-P2A-Neo was generated by mutating a pSIN vector backbone and the 5 LTR to remove BbsI sites and assembling a hU6-BbsI-filler-BbsI-tracr EF1as-Thy1.1-P2A-Neo cassette downstream from the packaging signal
backbone_origin
backbone_size7099
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
targeting mouse Arkadia gene
inserts :
Stbl3
origin :
37
pi :
alt_names
RNF111
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterhuman U6
sequencing_primer_3
sequencing_primer_5LKO.1-5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesARK, Arkadia
geneRnf111
id93836
genbank_ids
mutation
nameArkadia targeting gRNA
shRNA_sequenceCATAGGATGCACCCAAACTA
size
species
10090
Mus musculus
tags
resistance markers :
295
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA