Circular 200,100 with interspersed loops (GAPDH) | Addgene 180183 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

Circular 200,100 with interspersed loops (GAPDH)

catalog :

180183

citations: 1

Reference
Katrekar D, Yen J, Xiang Y, Saha A, Meluzzi D, Savva Y, et al. Efficient in vitro and in vivo RNA editing via recruitment of endogenous ADARs using circular guide RNAs. Nat Biotechnol. 2022;40:938-945 pubmed publisher

product information

Catalog Number :

180183

Product Name :

Circular 200,100 with interspersed loops (GAPDH)

article :

doi	10.1038/s41587-021-01171-4
id	28216480
pubmed_id	35145312

bacterial resistance :

Kanamycin

cloning :

backbone

AAV

backbone_mutation

backbone_origin

Custom

backbone_size

6000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

AAV

growth strain :

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

origin :

pi :

sapienstags
loopsshRNA_sequencetd>GCCATCAGTCGCCGGTCCCAAGCCCGGATAAA
ATGGGAGGGGGCGGGAAACCGCCTAACCATGCCGACTGA
TGGCAGAAAAAAAAAAAGGCCATCCACAGTCTTCTGGGT
GGCAGTGATGGCATGGACTGTGGTCATGAGTCCTTCCAC
GATACGTTCGTTGTCATGGATGACCTTGGCCAGGGGTGC
CAAGCACAACGTGGTGCAGGAGGCATTGCTGATGATCTT
GAGGCTGTTGTCATACTTCTCATGGTTCACACCCATGAC
GAACATGGGGGCATCAGCAGAGAAAAAAAAAAAACTGCC
ATCAGTCGGCGTGGACTGTAGAACACTGCCAATGCCGGT
CCCAAGCCCGGATAAAAGTGGAGGGTACAGTCCACGCtd>size
species

9606

Ho
mo

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	Human U6
sequencing_primer_3
sequencing_primer_5	GACTATCATATGCTTACCGT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	G3PD, GAPD, HEL-S-162eP
gene	GAPDH
id	2597

genbank_ids

mutation

name

Circular 200,100 guide RNA with interspersed

resistance markers :

4273

tags :

Unknown