This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRB131
catalog :
180174
citations: 1
Reference
Cappelli J, Khacho P, Wang B, Sokolovski A, Bakkar W, Raymond S, et al. Glycine-induced NMDA receptor internalization provides neuroprotection and preserves vasculature following ischemic stroke. iScience. 2022;25:103539 pubmed publisher
product information
Catalog Number :
180174
Product Name :
pRB131
article :
doi10.1016/j.isci.2021.103539
id28223424
pubmed_id34977503
bacterial resistance :
Ampicillin
cloning :
backbonepAAV-CW3SL-EGFP
backbone_mutationEGFP dropped out by NheI and EcoRI. EcoRI site was blunt ended and GluN1 cloned in as a NheI EcoRV fragment.
backbone_originAddgene#61463
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
growth strain :
pAAV construct GluN1A714L (shRNA resistant)
origin :
30
pi :
alt_names
GriN1/ NMDAR1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5NheI
promoter
sequencing_primer_3GluN1 SeqR1 TGCAGGTTCTTCCTCCACAC
sequencing_primer_5GluN1 SeqF1 CATGGTGTGGGCTGGTTTCG
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesGluN1, NMDAR1, NR1
geneGrin1
id24408
genbank_ids
mutationA714L
nameGluN1
shRNA_sequence
size3545
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagPhluorin
resistance markers :
5792
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA