This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Flag-Bcl2-Cb5
catalog :
18004
citations: 3
Reference
Dulloo I, Atakpa Adaji P, Yeh Y, Levet C, Muliyil S, Lu F, et al. iRhom pseudoproteases regulate ER stress-induced cell death through IP3 receptors and BCL-2. Nat Commun. 2022;13:1257 pubmed publisher
Mughal W, Martens M, Field J, Chapman D, Huang J, Rattan S, et al. Myocardin regulates mitochondrial calcium homeostasis and prevents permeability transition. Cell Death Differ. 2018;25:1732-1748 pubmed publisher
Wang N, Unkila M, Reineks E, Distelhorst C. Transient expression of wild-type or mitochondrially targeted Bcl-2 induces apoptosis, whereas transient expression of endoplasmic reticulum-targeted Bcl-2 is protective against Bax-induced cell death. J Biol Chem. 2001;276:44117-28 pubmed
product information
Catalog Number :
18004
Product Name :
Flag-Bcl2-Cb5
article :
doi10.1074/jbc.M101958200
id2100
pubmed_id11546793
bacterial resistance :
Kanamycin
cloning :
backbonepCMV-Tag2B
backbone_mutation
backbone_originStratagene
backbone_size4300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Amino acids 100 134 (ITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED) human cytochrome b5 replaced the C-terminal domain of Bcl-2 (amino acids 219-239).
origin :
37
pi :
alt_names
Bcl-2 Cb5
Bcl2 Cytochrome b5
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterCMV
sequencing_primer_3T7
sequencing_primer_5T3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBcl-2, PPP1R50
geneBCL2
id596
genbank_ids
mutationC terminal of Bcl-2 replaced with Cytochrome b5. Targets Bcl-2 to the Endoplasmic Reticulum.
nameER targeted Bcl-2
shRNA_sequence
size761
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFlag
plasmid copy :
PCR amplified human Bcl-2 from pB4 plasmid purchased from ATCC. PCR amplified Cb5 from EST purchased from ATCC.
resistance markers :
117
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA