This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pT3EF5a-PIK3CA H1047R
catalog :
180034
citations: 1
Reference
Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, et al. Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to induce liver tumour formation in mice via AKT2/mTORC1 cascade. Liver Int. 2016;36:1176-86 pubmed publisher
product information
Catalog Number :
180034
Product Name :
pT3EF5a-PIK3CA H1047R
article :
doi10.1111/liv.13055
id17645
pubmed_id26716908
bacterial resistance :
Kanamycin
cloning :
backbonepENTR1A
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Entry Vector
growth notes :
This construct contains LoxP sequences flanking the pT3 sequences. Therefore, this plasmid could not be use in combination with CRE in the studies.
growth strain :
Entry vector of PIK3CA H1047R for gateway cloning.
origin :
37
pi :
alt_names
phosphoinositide 3-kinase
PI3K
p110 alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3GTAACATCAGAGATTTTGAGACAC
sequencing_primer_5CTACAAACTCTTCCTGTTAGTTA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
genePIK3CA
id5290
genbank_ids
NM_006218.4
mutationH1047R
namePIK3CA H1047R
shRNA_sequence
size3207
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA tag
plasmid copy :
HA-PIK3CAH1047R was cloned from Addgene #12524: pBabe-HA-PIK3CAH1047R
resistance markers :
1021
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA