This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pT3-EF1aH-PIK3CA H1047R
catalog :
180031
citations: 1
Reference
Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, et al. Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to induce liver tumour formation in mice via AKT2/mTORC1 cascade. Liver Int. 2016;36:1176-86 pubmed publisher
product information
Catalog Number :
180031
Product Name :
pT3-EF1aH-PIK3CA H1047R
article :
doi10.1111/liv.13055
id17645
pubmed_id26716908
bacterial resistance :
Ampicillin
cloning :
backbonepT3-EF1aH
backbone_mutation
backbone_origin
backbone_size7000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
origin :
37
pi :
alt_names
phosphoinositide 3-kinase
PI3K
p110 alpha
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3
sequencing_primer_5EF1a-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
genePIK3CA
id5290
genbank_ids
NM_006218.4
mutationH1047R
namePIK3CA H1047R
shRNA_sequence
size3207
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA tag
plasmid copy :
HA-PIK3CAH1047R was cloned from Addgene #12524: pBabe-HA-PIK3CAH1047R
resistance markers :
1021
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA