This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBPK1520-SNCA-A30P-ng
catalog :
180018
citations: 1
Reference
Li H, Busquets O, Verma Y, Syed K, Kutnowski N, Pangilinan G, et al. Highly efficient generation of isogenic pluripotent stem cell models using prime editing. elife. 2022;11: pubmed publisher
product information
Catalog Number :
180018
Product Name :
pBPK1520-SNCA-A30P-ng
article :
doi10.7554/eLife.79208
id28223409
pubmed_id36069759
bacterial resistance :
Ampicillin
cloning :
backboneBPK1520
backbone_mutation
backbone_originKeith Joung (Addgene plasmid # 65777)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsmBI
cloning_site_5BsmBI
promoterHuman U6
sequencing_primer_3
sequencing_primer_5hU6-F (GAGGGCCTATTTCCCATGATT)
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesNACP, PARK1, PARK4, PD1
geneSNCA
id6622
genbank_ids
mutation
namePrime editing ngRNA for SNCA-A30P
shRNA_sequenceACAAGCACCAAACTGACATT
size21
species
9606
Homo sapiens
tags
plasmid copy :
Backbone is from Keith Joung (Addgene plasmid # 65777)
resistance markers :
5785
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA