This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-Bcl2-Cb5
catalog :
18000
citations: 5
Reference
Lalier L, Mignard V, Joalland M, Lanoƫ D, Cartron P, Manon S, et al. TOM20-mediated transfer of Bcl2 from ER to MAM and mitochondria upon induction of apoptosis. Cell Death Dis. 2021;12:182 pubmed publisher
Castro Gonzalez S, Shi Y, Colomer Lluch M, Song Y, Mowery K, Almodovar S, et al. HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner. Autophagy. 2020;:1-25 pubmed publisher
Wu L, Xie J, Li T, Mai Z, Wang L, Wang X, et al. Gene delivery ability of polyethylenimine and polyethylene glycol dual-functionalized nanographene oxide in 11 different cell lines. R Soc Open Sci. 2017;4:170822 pubmed publisher
Kleemann B, Loos B, Scriba T, Lang D, Davids L. St John's Wort (Hypericum perforatum L.) photomedicine: hypericin-photodynamic therapy induces metastatic melanoma cell death. PLoS ONE. 2014;9:e103762 pubmed publisher
Wang N, Unkila M, Reineks E, Distelhorst C. Transient expression of wild-type or mitochondrially targeted Bcl-2 induces apoptosis, whereas transient expression of endoplasmic reticulum-targeted Bcl-2 is protective against Bax-induced cell death. J Biol Chem. 2001;276:44117-28 pubmed
product information
Catalog Number :
18000
Product Name :
GFP-Bcl2-Cb5
article :
doi10.1074/jbc.M101958200
id2100
pubmed_id11546793
bacterial resistance :
Kanamycin
cloning :
backbonepEMD-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Bcl2 amino acids 1-209 plus Cb5 amino acids 100 134 (ITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED).
origin :
37
pi :
alt_names
Bcl-2 Cb5
Bcl2 Cytochrome b5
cloning
clone_methodRestriction Enzyme
cloning_site_3Eco RI
cloning_site_5Bgl II
promoterCMV
sequencing_primer_3
sequencing_primer_5CATGGTCCTGCTGGAGTTCGT
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesBcl-2, PPP1R50
geneBCL2
id596
genbank_ids
mutationBcl-2's C terminal targeting sequence replaced with Cytochrome b5. Targets Bcl-2 to the Endoplasmic Reticulum.
nameER targeted Bcl-2
shRNA_sequence
size761
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP
plasmid copy :
PCR amplified human Bcl-2 from pB4 plasmid purchased from ATCC. PCR amplified Cb5 from EST purchased from ATCC.
resistance markers :
117
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA