This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJET-61-SFFV-T2A-PuroR-polyA-R11a
catalog :
179892
citations: 1
Reference
Gemperle J, Harrison T, Flett C, Adamson A, Caswell P. On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members. elife. 2022;11: pubmed publisher
product information
Catalog Number :
179892
Product Name :
pJET-61-SFFV-T2A-PuroR-polyA-R11a
article :
doi10.7554/eLife.76651
id28223385
pubmed_id35708998
bacterial resistance :
Ampicillin
cloning :
backbonepJet1.2
backbone_mutation
backbone_originThermo Scientific
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
CRISPR
Other
Donor for homologous based knock in
growth strain :
Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out
origin :
37
pi :
alt_names
YL8
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterSFFV
sequencing_primer_3AAGAACATCGATTTTCCATGGCAG
sequencing_primer_5CGACTCACTATAGGGAGAGCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYL8
geneRAB11A
id8766
genbank_ids
mutation
nameRAB11A, member RAS oncogene family
shRNA_sequence
size1898
species
9606
Homo sapiens
tags
resistance markers :
5810
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA