pCDH-AtAFB2-mTagBFP2 | Addgene 179889 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCDH-AtAFB2-mTagBFP2

catalog :

179889

citations: 1

Reference
Gemperle J, Harrison T, Flett C, Adamson A, Caswell P. On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members. elife. 2022;11: pubmed publisher

product information

Catalog Number :

179889

Product Name :

pCDH-AtAFB2-mTagBFP2

article :

doi	10.7554/eLife.76651
id	28223385
pubmed_id	35708998

bacterial resistance :

Ampicillin

cloning :

backbone

pCDH

backbone_mutation

backbone_origin

Systembio

backbone_size

6390

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Bacterial Expression

Lentiviral

growth notes :

This lentiviral plasmids delivers AtAFB2 (auxin-inducible degron system) fused to mTagBFP2. In combination with TetOn3G can be used to generate DExogron = DExCon (Doxycycline-mediated endogenous gene Expression Control) combined with auxin-mediated targeted protein degradation. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.

growth strain :

Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)

origin :

pi :

alt_names

AFB2

AT3G26810

auxin signaling F-box 2

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	EF1
sequencing_primer_3
sequencing_primer_5	CTCCACGCTTTGCCTGACCCTGCTT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT3G26810, auxin signaling F-box 2
gene	AFB2
id	822296

genbank_ids

MP787830.1

mutation

name

AtAFB2

shRNA_sequence

size

2450

species

3702

Arabidopsis thaliana

100

Synthetic