Multiplex-CRISPR-miR-200FAM-T2A-GFP | Addgene 179856 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Multiplex-CRISPR-miR-200FAM-T2A-GFP

catalog :

179856

citations: 1

Reference
Yu L, Cao C, Li X, Zhang M, Gu Q, Gao H, et al. Complete loss of miR-200 family induces EMT associated cellular senescence in gastric cancer. Oncogene. 2022;41:26-36 pubmed publisher

product information

Catalog Number :

179856

Product Name :

Multiplex-CRISPR-miR-200FAM-T2A-GFP

article :

doi	10.1038/s41388-021-02067-y
id	28223374
pubmed_id	34667277

bacterial resistance :

Ampicillin

cloning :

backbone

pLV hUbC-Cas9-T2A-GFP

backbone_mutation

backbone_origin

Charles Gersbach (Addgene plasmid # 53190)

backbone_size

10000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

CRISPR

growth notes :

Oligonucleotides for cloning protospacers: h200b-mU6-O1 TTGTTTGGCCATCTTACTGGGCAGCAT h200b-mU6-O2 AAACATGCTGCCCAGTAAGATGGCCAA h429-hU6-O1 CACCGGCCCTCTGTCTAATACTGTC h429-hU6-O2 AAACGACAGTATTAGACAGAGGGCC h200c-7SK-O1 CCTCGTCGTCTTACCCAGCAGTGTT h200c-7SK-O2 AAACAACACTGCTGGGTAAGACGAC h141-H1-O1 TCCCATCCAACACTGTACTGGAAGA h141-H1-O2 AAACTCTTCCAGTACAGTGTTGGAT

growth strain :

to delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200b

origin :

pi :

alt_names

cloning

entrez_gene

aliases	MIRN141, mir-141
gene	MIR141
id	406933

aliases	MIRN200B, mir-200b
gene	MIR200B
id	406984

aliases	MIRN429, hsa-mir-429, mir-429
gene	MIR429
id	554210

aliases	MIRN200C, mir-200c
gene	MIR200C
id	406985

aliases	MIRN200A, mir-200a
gene	MIR200A
id	406983

genbank_ids

miR-200 family: Homo sapiens miR-200b, LM608401.1, Homo sapiens miR-429, LM379507.1, Homo sapiens miR-141: LM379050.1

Homo sapiens miR-200c: LM608608.1, Homo sapiens miR-200a: LM608664.1

mutation

name

hsa-miR-200 family (miR-200b, miR-200a, miR-429, miR-141 and miR-200c )

shRNA_sequence

gRNA sequence details listed in Supplementary Information Table S4 of the Article and below.

size

species

9606

Homo sapiens