This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJH2198
catalog :
179773
citations: 1
Reference
Xie L, Gao S, Alcaire S, Aoyagi K, Wang Y, Griffin J, et al. NLF-1 delivers a sodium leak channel to regulate neuronal excitability and modulate rhythmic locomotion. Neuron. 2013;77:1069-82 pubmed publisher
product information
Catalog Number :
179773
Product Name :
pJH2198
article :
doi10.1016/j.neuron.2013.01.018
id28223364
pubmed_id23522043
bacterial resistance :
Ampicillin
cloning :
backbonepBSK
backbone_mutation
backbone_originstratagene
backbone_size7441
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth notes :
Please note Addgene NGS identified 3 mutations in the exons- I51N (exon1), T39A, L233S (exon2). Depositor confirms mutations do not affect plasmid function.
growth strain :
Prgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFP
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5BamHI
promoterPrgef-1
sequencing_primer_3CTATAACAACAACATAA
sequencing_primer_5ATGCGGCCTCGCACGCCT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_F55A4.2
genenlf-1
id180454
genbank_ids
mutation
nameNLF-1::GFP
shRNA_sequence
size2271
species
6239
Caenorhabditis elegans
tags
location
tagGFP
resistance markers :
4617
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA