This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJH2028
catalog :
179771
citations: 1
Reference
Xie L, Gao S, Alcaire S, Aoyagi K, Wang Y, Griffin J, et al. NLF-1 delivers a sodium leak channel to regulate neuronal excitability and modulate rhythmic locomotion. Neuron. 2013;77:1069-82 pubmed publisher
product information
Catalog Number :
179771
Product Name :
pJH2028
article :
doi10.1016/j.neuron.2013.01.018
id28223364
pubmed_id23522043
bacterial resistance :
Ampicillin
cloning :
backbonepBSK
backbone_mutation
backbone_originstratagene
backbone_size5089
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth notes :
Please note Addgene NGS found some differences in genomic sequence of NLF-1. Depositor confirms this does not affect plasmid function.
growth strain :
Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of NLF::2XGFP
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoternlf-1
sequencing_primer_3CTATAACAACAACATA
sequencing_primer_5ATGCGGCCTCGCACGCCTTA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_F55A4.2
genenlf-1
id180454
genbank_ids
mutation
nameNLF-1::2XGFP
shRNA_sequence
size5317
species
6239
Caenorhabditis elegans
tags
location
tag2XGFP
resistance markers :
4617
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA