This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDH-TfR-mApple
catalog :
179384
citations: 1
Reference
Rennick J, Nowell C, Pouton C, Johnston A. Resolving subcellular pH with a quantitative fluorescent lifetime biosensor. Nat Commun. 2022;13:6023 pubmed publisher
product information
Catalog Number :
179384
Product Name :
pCDH-TfR-mApple
article :
doi10.1038/s41467-022-33348-z
id28223297
pubmed_id36224168
bacterial resistance :
Ampicillin
cloning :
backbonepCDH
backbone_mutation
backbone_originSystem Biosciences
backbone_size7644
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Please visit https://doi.org/10.1101/2022.04.13.488146 for bioRxiv preprint.
growth strain :
Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterEF1
sequencing_primer_3gcttcggccagtaacgtta
sequencing_primer_5caagctgtgaccggcg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90
geneTFRC
id7037
genbank_ids
7037
mutation
nameTransferrin receptor 1 - mApple
shRNA_sequence
size3039
species
9606
Homo sapiens
100
Synthetic
tags
locationC terminal on insert
tagmApple
plasmid copy :
mApple and TfR DNA were obtained from mApple-Lysosomes-20 (RRID:Addgene_54921) and mCherry-TFR-20 (RRID:Addgene_55144) respectively, which were a gift from Michael Davidson.
resistance markers :
4468
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA