This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCAG-mPpp3cc-FLAG-6XHIS
catalog :
179160
citations: 1
Reference
Miyata H, Satouh Y, Mashiko D, Muto M, Nozawa K, Shiba K, et al. Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science. 2015;350:442-5 pubmed publisher
product information
Catalog Number :
179160
Product Name :
pCAG-mPpp3cc-FLAG-6XHIS
article :
doi10.1126/science.aad0836
id28203646
pubmed_id26429887
bacterial resistance :
Ampicillin
cloning :
backbonepCAG1.1
backbone_mutationpCAGGS was used as an original vector.
backbone_origin
backbone_size5160
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.
origin :
37
pi :
alt_names
Ppp3cc
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3GCCACACCAGCCACCACCTTCTG
sequencing_primer_5GCCTTCTTCTTTTTCCTACAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCalnc, PP2BA gamma
genePpp3cc
id19057
genbank_ids
mutation
nameprotein phosphatase 3, catalytic subunit, gamma isoform
shRNA_sequence
size1610
species
10090
Mus musculus
tags
locationC terminal on insert
tagFLAG
locationC terminal on insert
tag6XHIS
resistance markers :
1801
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA