This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCAG-hSPATA33-8xHIS-1D4
catalog :
179133
citations: 1
Reference
Miyata H, Oura S, Morohoshi A, Shimada K, Mashiko D, Oyama Y, et al. SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
product information
Catalog Number :
179133
Product Name :
pCAG-hSPATA33-8xHIS-1D4
article :
doi10.1073/pnas.2106673118
id28223251
pubmed_id34446558
bacterial resistance :
Ampicillin
cloning :
backbonepCAG1.1
backbone_mutationpCAGGS was used as an original vector. 8XHIS and 1D4 sequences were added.
backbone_origin
backbone_size5232
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression vector of human spermatogenesis associated 33 (SPATA33) tagged with 8XHIS and 1D4 at C-terminus, CAG promoter, rabbit globin poly(A) signal.
origin :
37
pi :
alt_names
SPATA33
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XbaI
promoter
sequencing_primer_3GCCACACCAGCCACCACCTTCTG
sequencing_primer_5GCCTTCTTCTTTTTCCTACAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC16orf55
geneSPATA33
id124045
genbank_ids
mutation
namespermatogenesis associated 33
shRNA_sequence
size429
species
9606
Homo sapiens
tags
locationC terminal on backbone
tag8XHIS
locationC terminal on backbone
tag1D4
resistance markers :
1801
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA