This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Flag-SIRT1
catalog :
1791
citations: 30
Reference
Kim G, Bhattarai P, Lim S, Lee K, Choi H. Sirtuin 5-mediated deacetylation of TAZ at K54 promotes melanoma development. Cell Oncol (Dordr). 2023;: pubmed publisher
Yu W, Venkatraman A, Menden H, Martinez M, Umar S, Sampath V. Short-chain fatty acids ameliorate necrotizing enterocolitis-like intestinal injury through enhancing Notch1-mediated single immunoglobulin interleukin-1-related receptor, toll-interacting protein, and A20 induction. Am J Physiol Gastrointest Liver Physiol. 2023;324:G24-G37 pubmed publisher
Yang Y, Zhang S, Guan J, Jiang Y, Zhang J, Luo L, et al. SIRT1 attenuates neuroinflammation by deacetylating HSPA4 in a mouse model of Parkinson's disease. Biochim Biophys Acta Mol Basis Dis. 2022;1868:166365 pubmed publisher
Lee Y, Kim S, Fang X, Song N, Kim D, Suh J, et al. JNK-mediated Ser27 phosphorylation and stabilization of SIRT1 promote growth and progression of colon cancer through deacetylation-dependent activation of Snail. Mol Oncol. 2022;16:1555-1571 pubmed publisher
Chen Y, Jiang Y, Yang Y, Huang X, Sun C. SIRT1 Protects Dopaminergic Neurons in Parkinson's Disease Models via PGC-1α-Mediated Mitochondrial Biogenesis. Neurotox Res. 2021;: pubmed publisher
Sung J, Kim S, Kim J, Choi H. Prednisolone suppresses adriamycin-induced vascular smooth muscle cell senescence and inflammatory response via the SIRT1-AMPK signaling pathway. PLoS ONE. 2020;15:e0239976 pubmed publisher
Li X, Li D, Liu C, Zhang M, Guan X, Fu Y. p33ING1b regulates acetylation of p53 in oral squamous cell carcinoma via SIR2. Cancer Cell Int. 2020;20:398 pubmed publisher
Sung J, Kim S, Cho D, Kim J, Choi H. SRT1720-induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA-dependent phosphorylation of AMPKα at Ser485. FEBS Open Bio. 2020;: pubmed publisher
Bayele H. A conserved mechanism of sirtuin signalling through steroid hormone receptors. Biosci Rep. 2019;39: pubmed publisher
Yuan F, Wang J, Li R, Zhao X, Zhang Y, Liu B, et al. A New Regulatory Mechanism Between P53 And YAP Crosstalk By SIRT1 Mediated Deacetylation To Regulate Cell Cycle And Apoptosis In A549 Cell Lines. Cancer Manag Res. 2019;11:8619-8633 pubmed publisher
Mu N, Lei Y, Wang Y, Wang Y, Duan Q, Ma G, et al. Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis. 2019;24:798-811 pubmed publisher
Weston S, Matthews K, Lent R, Vlk A, Haupt R, Kingsbury T, et al. A yeast suppressor screen used to identify mammalian SIRT1 as a proviral factor for MERS-CoV replication. J Virol. 2019;: pubmed publisher
Dhar S, Zhao D, Lin T, Gu B, Pal K, Wu S, et al. MLL4 Is Required to Maintain Broad H3K4me3 Peaks and Super-Enhancers at Tumor Suppressor Genes. Mol Cell. 2018;70:825-841.e6 pubmed publisher
Hong Q, Zhang L, Das B, Li Z, Liu B, Cai G, et al. Increased podocyte Sirtuin-1 function attenuates diabetic kidney injury. Kidney Int. 2018;93:1330-1343 pubmed publisher
Singh P, Hanson P, Morris C. SIRT1 ameliorates oxidative stress induced neural cell death and is down-regulated in Parkinson's disease. BMC Neurosci. 2017;18:46 pubmed publisher
Das D, Smith N, Wang X, Morgan I. The Deacetylase SIRT1 Regulates the Replication Properties of Human Papillomavirus 16 E1 and E2. J Virol. 2017;91: pubmed publisher
Soriano Arroquia A, House L, Tregilgas L, Canty Laird E, Goljanek Whysall K. The functional consequences of age-related changes in microRNA expression in skeletal muscle. Biogerontology. 2016;17:641-54 pubmed publisher
Zhang W, Luo J, Yang F, Wang Y, Yin Y, Strom A, et al. BRCA1 inhibits AR-mediated proliferation of breast cancer cells through the activation of SIRT1. Sci Rep. 2016;6:22034 pubmed publisher
Pinton G, Zonca S, Manente A, Cavaletto M, Borroni E, Daga A, et al. SIRT1 at the crossroads of AKT1 and ERβ in malignant pleural mesothelioma cells. Oncotarget. 2016;7:14366-79 pubmed publisher
Pasquel D, Doricakova A, Li H, Kortagere S, Krasowski M, Biswas A, et al. Acetylation of lysine 109 modulates pregnane X receptor DNA binding and transcriptional activity. Biochim Biophys Acta. 2016;1859:1155-1169 pubmed publisher
Singh B, Sinha R, Zhou J, Tripathi M, Ohba K, Wang M, et al. Hepatic FOXO1 Target Genes Are Co-regulated by Thyroid Hormone via RICTOR Protein Deacetylation and MTORC2-AKT Protein Inhibition. J Biol Chem. 2016;291:198-214 pubmed publisher
Lee W, Lee W, Cheng C, Chen K, Chou C, Chung C, et al. Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1. Oncotarget. 2015;6:27580-95 pubmed publisher
Kleiner R, Verma P, Molloy K, Chait B, Kapoor T. Chemical proteomics reveals a γH2AX-53BP1 interaction in the DNA damage response. Nat Chem Biol. 2015;11:807-14 pubmed publisher
Ren Y, Du C, Yan L, Wei J, Wu H, Shi Y, et al. CTGF siRNA ameliorates tubular cell apoptosis and tubulointerstitial fibrosis in obstructed mouse kidneys in a Sirt1-independent manner. Drug Des Devel Ther. 2015;9:4155-71 pubmed publisher
Schuster S, Penke M, Gorski T, Petzold Quinque S, Damm G, Gebhardt R, et al. Resveratrol differentially regulates NAMPT and SIRT1 in Hepatocarcinoma cells and primary human hepatocytes. PLoS ONE. 2014;9:e91045 pubmed publisher
Chaudhuri A, Yelamanchili S, Fox H. MicroRNA-142 reduces monoamine oxidase A expression and activity in neuronal cells by downregulating SIRT1. PLoS ONE. 2013;8:e79579 pubmed publisher
Madabushi A, Hwang B, Jin J, Lu A. Histone deacetylase SIRT1 modulates and deacetylates DNA base excision repair enzyme thymine DNA glycosylase. Biochem J. 2013;456:89-98 pubmed publisher
Snider N, Leonard J, Kwan R, Griggs N, Rui L, Omary M. Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation. J Cell Biol. 2013;200:241-7 pubmed publisher
Tsai R, Discher D. Inhibition of "self" engulfment through deactivation of myosin-II at the phagocytic synapse between human cells. J Cell Biol. 2008;180:989-1003 pubmed publisher
Brunet A, Sweeney L, Sturgill J, Chua K, Greer P, Lin Y, et al. Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Science. 2004;303:2011-5 pubmed
product information
Catalog Number :
1791
Product Name :
Flag-SIRT1
article :
doi10.1126/science.1094637
id108
pubmed_id14976264
bacterial resistance :
Ampicillin
cloning :
backbonepECE
backbone_mutation
backbone_origin
backbone_size2877
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The HindIII site used for cloning this plasmid is not unique. There is an additional HindIII site at nucleotide 1569 of the NCBI Reference Sequence: NM_012238.4. There is a unique SacI site at the 5' end of the insert that could be used for cloning.
growth strain :
Mammalian expression of flag-tagged SIRT1
origin :
37
pi :
alt_names
Sir2
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5SV40pro-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSIR2, SIR2L1, SIR2alpha
geneSIRT1
id23411
genbank_ids
NM_012238
NP_036370
mutation
nameSIRT1
shRNA_sequence
size2314
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
174
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA