pCAGGS-chGrb2/eDHFR(69K6)-iRFP713 | Addgene 178854 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

catalog :

178854

citations: 1

Reference
Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, et al. A chemogenetic platform for controlling plasma membrane signaling and synthetic signal oscillation. Cell Chem Biol. 2022;29:1446-1464.e10 pubmed publisher

product information

Catalog Number :

178854

Product Name :

pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

article :

doi	10.1016/j.chembiol.2022.06.005
id	28216838
pubmed_id	35835118

bacterial resistance :

Ampicillin

cloning :

backbone

pCAGGS

backbone_mutation

backbone_origin

Dr. Jun-ichi Miyazaki (Osaka University, Japan)

backbone_size

4880

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.

growth strain :

Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CAG
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2
gene	GRB2
id	2885

genbank_ids

mutation

Grb2: Y160E

name

Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713

shRNA_sequence

size

1966

species

9606

Homo sapiens