This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMMP-MCS-IRES-eGFP-P2A-iCas9
catalog :
178533
citations: 1
Reference
Okamoto T, Natsume Y, Yamanaka H, Fukuda M, Yao R. A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids. STAR Protoc. 2021;2:100780 pubmed publisher
product information
Catalog Number :
178533
Product Name :
pCMMP-MCS-IRES-eGFP-P2A-iCas9
article :
doi10.1016/j.xpro.2021.100780
id28223175
pubmed_id34585151
bacterial resistance :
Ampicillin
cloning :
backbonepCMMP
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note: Plasmid contains F64L and V163A mutations in EGFP. These mutations are not known to affect plasmid function.
growth strain :
For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesAPAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56
geneCASP9
id842
genbank_ids
mutationFKBP12 F36 to V Truncated Caspase9 (135-416; lacking pro-domain)
nameiCaspase9
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
5740
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA