This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pETM33_SPSB1_SPRY
catalog :
178487
citations: 1
Reference
Benz C, Ali M, Krystkowiak I, Simonetti L, Sayadi A, Mihalic F, et al. Proteome-scale mapping of binding sites in the unstructured regions of the human proteome. Mol Syst Biol. 2022;18:e10584 pubmed publisher
product information
Catalog Number :
178487
Product Name :
pETM33_SPSB1_SPRY
article :
doi10.15252/msb.202110584
id28223170
pubmed_id35044719
bacterial resistance :
Kanamycin
cloning :
backbonepETM33
backbone_mutation
backbone_origin
backbone_size6017
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
GDYCKPTRLDLLLDMPPVSYDVQLLHSWNNNDRSLNVFV
KEDDKLIFHRHPVAQSTDAIRGKVGYTRGLHVWQITWAM
RQRGTHAVVGVATADAPLHSVGYTTLVGNNHESWGWDLG
RNRLYHDGKNQPSKTYPAFLEPDETFIVPDSFLVALDMD
DGTLSFIVDGQYMGVAFRGLKGKKLYPVVSAVWGHCEIR
MRYLNGLD.
Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439572v1 for bioRxv preprint. Growth in BL21 DE3 gold 37 C after induction with 1mM IPTG 4 hours 30 C. Insert:
growth strain :
Bacterial expression of human domain with His-tag and GST tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5Nco1
promoterT7/LacO
sequencing_primer_3GAGTGCGGCCGCAAGCTTG
sequencing_primer_5CGGATCTGGAAGTTCTGTTCC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesSSB-1, SSB1
geneSPSB1
id80176
genbank_ids
mutation
nameSPSB1_SPRY
shRNA_sequence
size
species
9606
Homo sapiens
32644
Other
Synthetic
tags
locationN terminal on insert
tagHis-GST
resistance markers :
5021
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA