This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA-Mirk (YF)
catalog :
17845
citations: 2
Reference
Chang C, Chiu C, Liu P, Wu C, Tseng Y, Lee C, et al. Kinome-Wide siRNA Screening Identifies DYRK1B as a Potential Therapeutic Target for Triple-Negative Breast Cancer Cells. Cancers (Basel). 2021;13: pubmed publisher
Lee K, Deng X, Friedman E. Mirk protein kinase is a mitogen-activated protein kinase substrate that mediates survival of colon cancer cells. Cancer Res. 2000;60:3631-7 pubmed
product information
Catalog Number :
17845
Product Name :
pcDNA-Mirk (YF)
article :
doi
id2067
pubmed_id10910078
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1/HisA
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The insert is an StuI fragment of the mirk/dyrk1b gene. The EcoRV site was destroyed during cloning, but a new BamHI site was generated at the 3' end.
origin :
37
pi :
alt_names
Dyrk1B
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRV
cloning_site_5EcoRV
promoter
sequencing_primer_3BGH rev
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesAOMS3, MIRK
geneDYRK1B
id9149
genbank_ids
AAF15893
mutationY271/273F
nameMirk
shRNA_sequence
size2000
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6xHis
locationN terminal on backbone
tagAnti-XPress
resistance markers :
150
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA