This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSecTag-ATX
catalog :
17839
citations: 3
| Reference |
|---|
Jethwa S, Leah E, Zhang Q, Bright N, Oxley D, Bootman M, et al. Exosomes bind to autotaxin and act as a physiological delivery mechanism to stimulate LPA receptor signalling in cells. J Cell Sci. 2016;129:3948-3957 pubmed
|
product information
Catalog Number :
17839
Product Name :
pSecTag-ATX
article :
| doi | 10.1038/ni1573 |
| id | 2059 |
| pubmed_id | 18327261 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pSecTag | |
| backbone_mutation | ||
| backbone_origin | Invitrogen | |
| backbone_size | 5200 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
From the depositor: In 1998, we purchased from Invitrogen pSecTag2A and cut it with BglII and BamH1. The BglII/BamH1 fragment was replaced with a modified fragment containing 6 Histidines at the N-Terminus that was done by generating the modification by PCR, and cloning the PCR product back into BglII/BamH1. Sometime later, the above pSecTag2A-6HIS was digested with BamH1and Not1. The ATX (amino acids 52-863) insert was genereated by PCR, digested with BamH1/EcoR1 and by excising ATX from the original colony II cDNA library with EcoR1/Not1, which yields two pieces in two stages into the vector. Their is a stop codon before the C-Terminus Myc tag so it will not be expressed.
origin :
37
pi :
|
resistance markers :
| 424 |
tags :
High Copy
terms :
| Zeocin |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments