This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pACEBac1-gamma-tubulin(N229A)-TEV-His6
catalog :
178077
citations: 1
Reference
Wieczorek M, Ti S, Urnavicius L, Molloy K, Aher A, Chait B, et al. Biochemical reconstitutions reveal principles of human γ-TuRC assembly and function. J Cell Biol. 2021;220: pubmed publisher
product information
Catalog Number :
178077
Product Name :
pACEBac1-gamma-tubulin(N229A)-TEV-His6
article :
doi10.1083/jcb.202009146
id28223102
pubmed_id33496729
bacterial resistance :
Gentamicin
cloning :
backbonepACEBac1
backbone_mutation
backbone_originGeneva Biotech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
and GACATGATGGTAGACACCAGCTGGGCGATCTGGGAGAAGGATGGG
CCCATCCTTCTCCCAGATCGCCCAGCTGGTGTCTACCAT
CATGTC
Primers used to generate pACEBac1-gamma-tubulin(N229A)-TEV-His6 via site-directed mutagenesis were
growth strain :
Insect cell expression of gamma-tubulin(N229A)-TEV-His6
origin :
37
pi :
alt_names
TUBG1
cloning
entrez_gene
aliasesCDCBM4, GCP-1, TUBG, TUBGCP1
geneTUBG1
id7283
genbank_ids
mutationChanged Asparagine 229 to Alanine
namegamma-tubulin with an N229A point mutation
shRNA_sequence
size1353
species
9606
Homo sapiens
tags
locationC terminal on insert
tagTEV-His6
plasmid copy :
Originally published in Murphy et al., 2001 and received from Dr. Jens Luders (Institute for Research in Biomedicine, Barcelona, Spain)
resistance markers :
5094
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA