This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSCV Cdk2ap1CAN-HA
catalog :
178030
citations: 1
Reference
Modzelewski A, Shao W, Chen J, Lee A, Qi X, Noon M, et al. A mouse-specific retrotransposon drives a conserved Cdk2ap1 isoform essential for development. Cell. 2021;184:5541-5558.e22 pubmed publisher
product information
Catalog Number :
178030
Product Name :
pMSCV Cdk2ap1CAN-HA
article :
doi10.1016/j.cell.2021.09.021
id28223096
pubmed_id34644528
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV PIG (Puro IRES GFP empty vector)
backbone_mutation
backbone_originDavid Bartel
backbone_size7657
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Retroviral vector for the purpose of overexpression in mammalian cell culture
origin :
37
pi :
alt_names
Cdk2ap1 Chimeric
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRi
cloning_site_5XHO1
promoterGAG
sequencing_primer_3gagacgtgctacttccatttgt
sequencing_primer_5cccttgaacctcctcgttcgacc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesApc10, Cdkap1, DORC1, Doc1, ST19, doc-1, p12
geneCdk2ap1
id13445
genbank_ids
13445
mutationFirst N-Terminal 27aa are deleted
nameCdk2ap1
shRNA_sequence
size342
species
10090
Mus musculus
tags
locationC terminal on insert
tagHA
resistance markers :
711
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA