CoV2-M-IRES-E | Addgene 177938 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CoV2-M-IRES-E

catalog :

177938

citations: 5

Reference
Lusvarghi S, Stauft C, Vassell R, Williams B, Baha H, Wang W, et al. Effects of N-glycan modifications on spike expression, virus infectivity, and neutralization sensitivity in ancestral compared to Omicron SARS-CoV-2 variants. PLoS Pathog. 2023;19:e1011788 pubmed publisher
Liu H, Liu D. Development of novel SARS-CoV-2 viral vectors. Sci Rep. 2023;13:13053 pubmed publisher
Sengar A, Cervantes M, Bondalapati S, Hess T, Kasson P. Single-Virus Fusion Measurements Reveal Multiple Mechanistically Equivalent Pathways for SARS-CoV-2 Entry. J Virol. 2023;97:e0199222 pubmed publisher
Palermo E, Alexandridi M, Di Carlo D, Muscolini M, Hiscott J. Virus-like particle - mediated delivery of the RIG-I agonist M8 induces a type I interferon response and protects cells against viral infection. Front Cell Infect Microbiol. 2022;12:1079926 pubmed publisher
Syed A, Taha T, Tabata T, Chen I, Ciling A, Khalid M, et al. Rapid assessment of SARS-CoV-2-evolved variants using virus-like particles. Science. 2021;374:1626-1632 pubmed publisher

product information

Catalog Number :

177938

Product Name :

CoV2-M-IRES-E

article :

doi	10.1126/science.abl6184
id	28220280
pubmed_id	34735219

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please visit https://www.biorxiv.org/content/10.1101/2021.08.05.455082v1 for bioRxv preprint.

growth strain :

Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GU280_gp04
gene	E
id	43740570

aliases	GU280_gp05
gene	M
id	43740571

genbank_ids

mutation

name

SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence)

shRNA_sequence

size

species

32644

Other