This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28a(His10-PD-1cyto)
catalog :
177896
citations: 1
Reference
Hui E, Cheung J, Zhu J, Su X, Taylor M, Wallweber H, et al. T cell costimulatory receptor CD28 is a primary target for PD-1-mediated inhibition. Science. 2017;355:1428-1433 pubmed publisher
product information
Catalog Number :
177896
Product Name :
pET28a(His10-PD-1cyto)
article :
doi10.1126/science.aaf1292
id28223080
pubmed_id28280247
bacterial resistance :
Kanamycin
cloning :
backbonepET28A
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
E coli expression of His10 tag fused to human PD-1 Intracellular Domain
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5unknown
promoterT7
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1
genePDCD1
id5133
genbank_ids
mutation
namePD-1 (aa 194-288 only)
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tag10xHis-Thrombin cut site-T7 tag
resistance markers :
543
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA