This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)
catalog :
177887
citations: 1
Reference
Hui E, Vale R. In vitro membrane reconstitution of the T-cell receptor proximal signaling network. Nat Struct Mol Biol. 2014;21:133-42 pubmed publisher
product information
Catalog Number :
177887
Product Name :
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)
article :
doi10.1038/nsmb.2762
id28223079
pubmed_id24463463
bacterial resistance :
Ampicillin
cloning :
backbonepFastBacHT
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5unknown
promoterPolyhedrin
sequencing_primer_3CAAATGTGGTATGGCTGATT
sequencing_primer_5GGATTATTCATACCGTCCCA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesIMD22, LSK, YT16, p56lck, pp58lck
geneLCK
id3932
genbank_ids
mutationG2A
nameLck
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tag10xHis-TEV-4xFRB-SNAP tag
resistance markers :
543
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA