This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
L4440_BioBrick-hsf-1-sgRNA-A
catalog :
177786
citations: 1
Reference
Fischer F, Benner C, Goyala A, Grigolon G, Vitiello D, Wu J, et al. Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation. J Biol Chem. 2022;298:102085 pubmed publisher
product information
Catalog Number :
177786
Product Name :
L4440_BioBrick-hsf-1-sgRNA-A
article :
doi10.1016/j.jbc.2022.102085
id28228824
pubmed_id35636511
bacterial resistance :
Ampicillin
cloning :
backboneL4440_BioBrick-sgRNA
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)
origin :
37
pi :
and CCGGGAGAGGAGGAGCTCCGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTTsizespecies
6239
Caenorhabditis elegans
tags
Enzymecloning_site_3<
/td>Unknownclonin
g_site_5Unknown<
td>promoters
equencing_primer_3r>sequencing_primer_5Unkno
wnsite_3_destroyedd>site_5_destroye
d<
tr>entrez_gene<
td>
d>hsf-1
aliasesCELE_
Y53C10A.12
gene
id173
078

genbank_ids<
/td>mutation
namehsf-1r>shRNA_sequenceGATATG
TTTCTTGAGATCAGGTTTTAGAGCTAGAAATAGCAAGTT
AAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCA
CCGAGTCGGTGCTTTTTTT
alt_names
cloning
clone_methodRestriction
resistance markers :
418
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA