L4440_BioBrick-hif-1-sgRNA-A | Addgene 177784 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

L4440_BioBrick-hif-1-sgRNA-A

catalog :

177784

citations: 1

Reference
Fischer F, Benner C, Goyala A, Grigolon G, Vitiello D, Wu J, et al. Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation. J Biol Chem. 2022;298:102085 pubmed publisher

product information

Catalog Number :

177784

Product Name :

L4440_BioBrick-hif-1-sgRNA-A

article :

doi	10.1016/j.jbc.2022.102085
id	28228824
pubmed_id	35636511

bacterial resistance :

Ampicillin

cloning :

backbone

L4440_BioBrick-sgRNA

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

origin :

pi :

and AGGACACAAAAAAACTATGGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTTsizespecies

6239

Caenorhabditis elegans

tags
Enzymecloning_site_3<
/td>Unknownclonin
g_site_5Unknown<
td>promoters
equencing_primer_3r>sequencing_primer_5Unkno
wnsite_3_destroyedd>site_5_destroye
d<
tr>entrez_gene<
td>

aliases	CELE_ F38A6.3
gene	h if-1
id	180359

d>genbank_ids>mutationr>namehif-1<
tr>shRNA_sequenceAAAAGGACA
CAAAAAAACTACGTTTTAGAGCTAGAAATAGCAAGTTAA
AATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACC
GAGTCGGTGCTTTTTTT

alt_names

cloning

clone_method

Restriction

resistance markers :

418

tags :

High Copy