This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pT7C-HSPA8
catalog :
177661
citations: 1
Reference
Ayoub C, Wagner C, Kuret J. Identification of gene networks mediating regional resistance to tauopathy in late-onset Alzheimer's disease. PLoS Genet. 2023;19:e1010681 pubmed publisher
product information
Catalog Number :
177661
Product Name :
pT7C-HSPA8
article :
doi10.1371/journal.pgen.1010681
id28223029
pubmed_id36972319
bacterial resistance :
Ampicillin
cloning :
backbonepT7C
backbone_mutation
backbone_origin
backbone_size3352
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Genes were cloned from a cDNA library and may contain silent mutations. However, all inserts were sequence-verified and any nucleotide changes were mutagenized to encode the consensus amino acid sequence.
growth strain :
Recombinant protein synthesis of human HSPA8
growth temp :
To produce protein, express in BL21(DE3) Codon Plus RIL. Purify by Ni IMAC and SEC.
origin :
37
pi :
alt_names
NP_006588.1
HSPA8 Isoform 1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5SpeI
promoterT7
sequencing_primer_3GATTATCAACCGGGGTGGCA
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
geneHSPA8
id3312
genbank_ids
mutation
nameHSPA8
shRNA_sequence
size1941
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6xHis-Thrombin
resistance markers :
5703
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA