This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pT7II-2N4Rtau
catalog :
177653
citations: 1
Reference
Ayoub C, Wagner C, Kuret J. Identification of gene networks mediating regional resistance to tauopathy in late-onset Alzheimer's disease. PLoS Genet. 2023;19:e1010681 pubmed publisher
product information
Catalog Number :
177653
Product Name :
pT7II-2N4Rtau
article :
doi10.1371/journal.pgen.1010681
id28223029
pubmed_id36972319
bacterial resistance :
Ampicillin
cloning :
backbonepT7II
backbone_mutation
backbone_origin
backbone_size3256
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Genes were cloned from a cDNA library and may contain silent mutations. However, all inserts were sequence-verified and any nucleotide changes were mutagenized to encode the consensus amino acid sequence.
growth strain :
Recombinant protein synthesis of human Tau
growth temp :
To produce tau protein, express in BL21(DE3) Codon Plus RP. Purify by Ni IMAC and SEC.
origin :
37
pi :
alt_names
NP_005901.2
MAPT Isoform 2
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5NdeI
promoterT7
sequencing_primer_3GATTATCAACCGGGGTGGCA
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
geneMAPT
id4137
genbank_ids
mutation
nameTau (2N4R)
shRNA_sequence
size1326
species
9606
Homo sapiens
tags
resistance markers :
5703
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA