This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Tubp-Gal4 in pCaSpeR4
catalog :
17747
citations: 1
Reference
Lee T, Luo L. Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron. 1999;22:451-61 pubmed
product information
Catalog Number :
17747
Product Name :
Tubp-Gal4 in pCaSpeR4
article :
doi10.1016/S0896-6273(00)80701-1
id2038
pubmed_id10197526
bacterial resistance :
Ampicillin
cloning :
backbonepCaSpeR4
backbone_mutation
backbone_origin
backbone_size7855
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
The GAL4 gene containing the hsp70 polyA region was cut out with NotI from pGaTN (Brand and Perrimon, 1993) and inserted into pCaSpeR4 carrying the tubulin 1alpha promoter.
origin :
37
pi :
alt_names
Tubp-GAL4
GAL4 hsp70 polyA
hsp70 polyA
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI, BamHI?
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL248C, GAL81
geneGAL4
id855828
genbank_ids
GAL4
mutation
nameGAL4
shRNA_sequence
size5774
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagTubulin 1alpha promoter
locationC terminal on insert
taghsp70 PolyA
plasmid copy :
Tubulin 1alpha promoter fragment from Gary Struhl
resistance markers :
304
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA