This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCherry-Cb5-IRES-puromycin-pLVx-EF1a
catalog :
177434
product information
Catalog Number :
177434
Product Name :
mCherry-Cb5-IRES-puromycin-pLVx-EF1a
article :
doi
id28223012
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepLVX-EF1a-IRES-Puromycin
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Infection with this plasmid will express mCherry-fused to the N-terminus of Cb5 tail anchor sequence ITTVESNSSWWTNWVIPAISALVVALMYRLYMAED , which targets the cytoplasmic face of endoplasmic reticulum. There is a flexible, 7 aa linker SGLRSGK , between mCherry and Cb5.
growth strain :
To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.
origin :
37
pi :
alt_names
mCherry-Cb5, Ch-Cb5, ChCb5
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3
sequencing_primer_5aagcgcgatcacatggtcctg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNcb5or, b5&b5R, b5+b5R, cb5/cb5R
geneCyb5r4
id171015
genbank_ids
mutation
nameCb5 tail anchor
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagmCherry
plasmid copy :
See gene entry for cytochrome b5 [R. norvegicus] , See NCBI Reference Sequence: NP_071581.1. Here we have used only the C-terminal tail anchor sequence. mCherry sequence matches GenBank: AZP55984.1.
resistance markers :
4132
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA