This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCherry-ActA-IRES-puromycin-pLVx-EF1a
catalog :
177433
product information
Catalog Number :
177433
Product Name :
mCherry-ActA-IRES-puromycin-pLVx-EF1a
article :
doi
id28223012
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepLVX-EF1a-IRES-Puromycin
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Infection with this plasmid will express mCherry-fused to the N-terminus of ActA tail anchor sequence: LILAMLAIGVFSLGAFIKIIQLRKNN , which targets the mitochondrial outer membrane and signal correlates with mitotracker Green signal in cells. There is a flexible, 7 aa linker SGLRSRV , between mCherry and ActA.
growth strain :
To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
origin :
37
pi :
alt_names
mCherry-ActA, Ch-ActA, ChActA
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3
sequencing_primer_5aagcgcgatcacatggtcctg
site_3_destroyed
site_5_destroyed
entrez_gene
aliaseslmo0204
geneactA
id987035
genbank_ids
mutation
nameActA tail anchor
shRNA_sequence
size
species
32644
Other
Listeria monocytogenes
tags
locationN terminal on insert
tagmCherry
plasmid copy :
See gene entry for actin assembly-inducing protein ActA (NCBI Reference Sequence: WP_110138194.1). Here we have used only the C-terminal tail anchor sequence of this protein. mCherry sequence matches GenBank: AZP55984.1.
resistance markers :
4132
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA